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- 內容介紹
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Molecular Microbiology Laboratory
A Writing Intensive Course
Walt Ream , Oregon State University, Corvallis, U.S.A.
Bruce Geller , Oregon State University, Corvalis, Oregon, U.S.A.
Janine Trempy , Oregon State University, Corvalis, Oregon, U.S.A.
Katharine G. Field , Oregon State University
ISBN 012583991X · Paperback · 252 Pages
Academic Press · Published February 2003
Molecular Microbiology Laboratory is designed to teach molecular biology techniques to upper level undergraduates majoring in the life sciences. An extremely detailed lab preparation manual for teaching assistants accompanies the lab book and contains a general discussion of scientific writing and critical reading, as well as detailed instructions for preparation and peer review of lab reports. Each experimental unit is accompanied by a number of additional writing exercises based upon primary journal articles. The studies in these articles employ the techniques that the students are learning in the lab exercises, which reinforces their understanding of the material. These are techniques that students in any biological science will need to know, making this manual applicable to any life science curriculum.
Features
Not a typical cookbook lab exercise, offers students the excitement and intellectual challenge of characterizing true unknowns. They could discover a new species!
Success rate greater than 85% for the entire experiment, even with very inexperienced students. The ONLY manual that incorporates writing exercises into the curriculum.
Co-authored by Dr. Janine Trempy, one of four senior editors of the Journal of Microbiology Education, published by the American Society for Microbiology.
Contents
Introduction.
Experiment 1: Plasmid Purification and Restriction Mapping.
Experimant 2: Affinity Purification of Histidine-Tagged FnbA Protein.
Experimant 3: Polymerase Chain Reaction and DNA Sequence Analysis of Bacterial Ribosomal RNA Genes.
Experimant 4: Southern Blot Analysis of Bacterial rRNA Genes.
Appendix A.
Appendix B.
Appendix C.
Appendix D.
Molecular Microbiology Laboratory Preparation Manual.
Required and Suggested Readings.
Index.
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